If you can't delete an ISO file as the file is open in your System, check if it is mounted as a drive or not. Uses these proven fixes to resolve the issue.

If you cannot delete a file or a folder on an NTFS file system volume in Windows, follow the solutions mentioned in this post to resolve the issue.

Why Cant I Delete, Edit, or Rename a File That Is in Use? Windows prevents you from deleting, moving, or renaming files that are in use to keep your computer system stable.

Then I typed "fsutil reparsepoint delete <file name>" (without the quotes) then hit enter. I was then able to go back to the file and delete it normally. Not open for further replies.