DAPI is generally used to stain fixed cells since the dye is cell impermeant, although the stain will enter live cells when used at higher concentrations. For live-cell staining, Hoechst 33342 dye is a popular …

DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine – thymine -rich regions in DNA. It is used extensively in fluorescence microscopy.

Jul 16, 2025 · In this guide, you’ll learn the complete DAPI staining protocol, its uses, key precautions, and how to get consistent, reliable results every time. DAPI (4′,6-diamidino-2-phenylindole) is a blue …

Learn how DAPI binds to DNA, its fluorescence properties, and best practices for staining, imaging, and storage in laboratory applications.

We have developed an HPLC method for our DAPI products that shows reassay results from July 2012 for lots that were originally released for sale in December 2008, for both D9542 (DAPI …

Jun 10, 2025 · DAPI staining is a widely used method for visualising DNA in fixed or live cells, offering strong nuclear contrast in fluorescence microscopy. It binds specifically to A-T-rich regions in DNA, …

Aug 8, 2024 · Both DAPI and Hoechst are minor-groove binding dyes with a preference for A/T-rich regions of DNA over G/C-rich DNA. The dyes have minimal fluorescence in solution, but become …

Learn about DAPI staining for cell imaging. Explore its applications, benefits, and protocols to visualize DNA and enhance your fluorescence microscopy research.

A fluorescent stain that binds strongly to A-T rich regions in deoxyribonucleic acid (DNA) and can be used in fluorescence microscopy. As DAPI can pass through an intact cell membrane, it can be used …