Cre-lox Basics: Cre-lox Technology in Mouse Modeling

The Flp and Cre-lox site-specific recombination systems are wid... | Filo

SOLVED: Cre recombinase is a site-specific enzyme that catalyzes recombination between two LoxP DNA sites. Cre recombinase pairs two LoxP sites in the same orientation, breaks both duplexes at the same point in each LoxP site, and joins the ends with new partners so that each LoxP site is regenerated, as shown schematically in Figure Q5-4A. Based on this mechanism, predict the arrangement of sequences that will be generated by Cre-mediated site-specific recombination for each of the two DNAs sho

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